DNA microarray chip technology is a useful tool for investigating differentially-expressed genes in animals. Combining DNA microarray technology and polymorphism study, studies in our laboratory identified novel genetic markers of animals. These candidate gene markers offer more advantages in that they can be used for accurate animal selection and to rapidly improve performance over traditional breeding methods. Two examples of genotypes of novel genetic markers associated with hatchability (%) in Tsaiya ducks (Anas platyrhynchos) are shown in Table 1. A number of methods for genetic polymorphism analysis were employed, such as single-nucleotide polymorphism (SNP), random amplified polymorphic DNA (RAPD) [14-26], random amplified microsatellite polymorphism (RAMPO), amplified fragment length polymorphism (AFLP), single strand conformation polymorphism (SSCP), restriction fragment length polymorphism (RFLP) and mitochondrial DNA (mtDNA) sequence analyses. In the present paper, effective strategies and applications for identifying novel and stable genetic markers based on SNP, RAPD and RAMPO polymorphisms were investigated.
Author(s) Details:
Hsiu-Lin Huang,
Department of Animal Science, National Chung Hsing University, 145 Xingda Road, Taichung 402, Taiwan.
I-Yen Huang,
Ugintech Co. Ltd., 5F-5, No. 282, Zhishi North 2nd Road, Taichung 407, Taiwan.
Chia-Yu Lin,
Johnson Chemical Pharmaceutical Works Co. Ltd., No. 77, Sec. 4, Sanhe Road, Sanchong Dist., New Taipei City 241, Taiwan.
Mu-Chiou Huang,
Department of Animal Science, National Chung Hsing University, 145 Xingda Road, Taichung 402, Taiwan.
