DNA is degraded by enzymes like deoxyribonuclease (DNase), Restriction endonucleases, S-1 nuclease, exonucleases like Exo-1, Polymerase- δ, ε, γ, p53 and microbial contaminations. These enzymes are present ubiquitously as contaminant in several enzyme preparations and are also borne by contaminating micro-organisms. Nucleases make up 80% of the cell wall in Flavobacterium sp. like Staphylococcus aureus. It is also reported that the culture supernatant contained 31.3% of the total cellular DNase activity, while the cell wash, cell-wall compartmentalized and intracellular fractions contained 27.8%, 27.8% and 13.1%, respectively in Clostridium pasteurianum NRRL-B598. These enzymes are continuously released by every microbial cell for their self-defence.
Author(s) Details:
Lahiri S. S.,
Defence R & D Est., D.R.D.O., Gwalior, Madhya Pradesh, India and Institute of Nuclear Medicine and Allied Science (INMAS), DRDO, New Delhi, India and Amity University, Noida, Uttar Pradesh, India and Amity University, Noida, Uttar Pradesh, India.
Singh R.,
Defence R & D Est., D.R.D.O., Gwalior, Madhya Pradesh, India.
Karothia B. S.,
Defence R & D Est., D.R.D.O., Gwalior, Madhya Pradesh, India.
Giredhar M.,
Center for Bio-Hybrid Medical Systems, Forckenbeckstr. 55, 52074 Aachen, Germany.
Chakraborty A.,
Division of Immunology, Immunity to Infection and Respiratory Medicine, School of Biological Sciences, The University of Manchester, Manchester, M13 9PT, UK.
