DNA is Degraded by Enzymes Like Deoxyribonuclease: A Part from The Book Chapter : Homemade Molecular Devices, Detections and Methods

DNA is degraded by enzymes like deoxyribonuclease (DNase), Restriction endonucleases, S-1 nuclease, exonucleases like Exo-1, Polymerase- δ, ε, γ, p53 and microbial contaminations. These enzymes are present ubiquitously as contaminant in several enzyme preparations and are also borne by contaminating micro-organisms. Nucleases make up 80% of the cell wall in Flavobacterium sp. like Staphylococcus aureus. It is also reported that the culture supernatant contained 31.3% of the total cellular DNase activity, while the cell wash, cell-wall compartmentalized and intracellular fractions contained 27.8%, 27.8% and 13.1%, respectively in Clostridium pasteurianum NRRL-B598. These enzymes are continuously released by every microbial cell for their self-defence.

Author(s) Details:

Lahiri S. S.,
Defence R & D Est., D.R.D.O., Gwalior, Madhya Pradesh, India and Institute of Nuclear Medicine and Allied Science (INMAS), DRDO, New Delhi, India and Amity University, Noida, Uttar Pradesh, India and Amity University, Noida, Uttar Pradesh, India.

Singh R.,
Defence R & D Est., D.R.D.O., Gwalior, Madhya Pradesh, India.

Karothia B. S.,
Defence R & D Est., D.R.D.O., Gwalior, Madhya Pradesh, India.

Giredhar M.,
Center for Bio-Hybrid Medical Systems, Forckenbeckstr. 55, 52074 Aachen, Germany.

Chakraborty A.,
Division of Immunology, Immunity to Infection and Respiratory Medicine, School of Biological Sciences, The University of Manchester, Manchester, M13 9PT, UK.

To Read the Complete Chapter See Here

Related Posts

Terminalia chebula

Contraceptive Potential of Terminalia Chebula : A Part from the Book Chapter : Harad (Terminalia chebula Retz.): A Potential Candidate for Reversible Contraception in Male

Terminalia chebula (T. chebula; Image 1 A-E) Retz. (Family: Combretaeceae) carries several local names such as Black Myroblans (English), Haritaki (Sanskrit and Bengali), Harad (Hindi) etc., and…

Line Intercept Transect (LIT)

The Line Intercept Transect (LIT) method was applied to record coral on three transect lines : A Part from the Book Chapter : Assessing Planulae Recruitment in Degraded Coastal Coral of Panjang Island Jepara, Central Java-Indonesia

The Line Intercept Transect (LIT) method was applied to record coral on three transect lines of 30 m each held parallel to the coastline in each station,…

Bacillus thuringiensis

Several Subspecies of B. Thuringiensis have been Reported to Produce Chitinases : A Part from the Book Chapter : Genes chi of Native Bacillus thuringiensis Strains from Maranhão Active against Aedes aegypti Larvae

Several subspecies of B. thuringiensis have been reported to produce chitinases. Nowadays, there are more than 100 chitinase genes harbored in different subspecies of B. thuringiensis that…

Mitochondria

Mitochondrial ion Transport Mechanisms : A Part from the Book Chapter : Regulatory Mechanisms of Mitochondrial Volume in the Heart in Response to Physiological and Pathological Stimuli

Mitochondrial ion transport mechanisms are pivotal in the regulation of mitochondrial bioenergetics, primarily through the modulation of matrix Ca2+ levels. These changes in Ca2+ concentration directly influence…

Radiation exposure

Cancers is Proportional : A Part from the Book Chapter : Estimation of Low Radiation Doses Effects by Using Tumor Markers

The standard terminology for categorizing levels of radiation exposure was introduced by UNSCEAR in its 2012 publication. A low dose is classified as falling within the range…

S. jamaicensis

The presence of S.jamaicensis in Fields Grow With Other Plants : A Part from the Book Chapter : Determination of Plants Diversity and Similarity in Selected three Communities of Stachytarpheta jamaicensis (L.) Vahl

The presence of S.jamaicensis in fields grow with other plants either native or aliens plants in diverse composition, structure and dominance. These are influenced by environmental factors…

Leave a Reply