Untreated diabetic wounds provide an optimal environment for bacterial growth, which, over time, can develop resistance to common antibiotics and ultimately result in amputation. Therefore, it is necessary to search for new sources of antimicrobial molecules with wound healing activity owing to the presence of different secondary metabolites in medicinal plants.
Aims: This study was to evaluate the in vivo antibacterial and diabetic wound healing capabilities of 70° hydroethanolic extract of Eriosema robustum leaves on non-infected and infected diabetic wound.
Methodology: To do this, obese albino Wistar male rats (200–280 g) were divided into eleven groups and were made diabetic by intraperitoneal injection with a low dose of streptozotocin at 45 mg/kg of body weight. An excision wound with a surface area of 314 mm2 was created on the dorsal area of each animal, except in the uninjured diabetic group (UDG). The 70° hydroethanolic extract was used to prepare 1%, 5%, and 10% ointments, with L-Mesitran serving as the reference ointment. Healing potential was assessed by measuring wound contraction rates and determining serum and tissue hydroxyproline, serum lactate deydrogenase (LDH) and total protein levels (TP). The antibacterial power evaluated in vivo of Eriosema robustum leaves was also assessed by culturing the skin after healing.
Results: The results demonstrated a significantly faster healing rate in the non-infected groups (5%,10% and L-Mesitran) compared to the infected groups. The levels of tissue hydroxyproline and total proteins were significantly (p < 0.05) elevated in all treated groups compared to infected and negative controls, unlike serum hydroxyproline levels. LDH levels were significantly (p < 0.05) elevated in both negative control group compared to the treated groups. The culture of different skin samples on previously injured areas on the 20th day of treatment showed no growth of S. aureus on completely healed areas and a low rate in the groups treated during the healing process.
Conclusion: 70° Hydroethanolic leaves extract of Eriosema robustum possess in vivo
antibacterial activities and diabetic wound healing potential.
Author(s) Details:
Gael Marius Tsaffo
Research Unit of Microbiology and Antimicrobial Substances, Faculty of Science, University of Dschang, Dschang, Cameroon.
Richard S. Tagne
Department of Biomedical Sciences, Faculty of Science, The University of Ngaoundere, P.O. Box 454 Ngaoundere, Cameroon.
Steve Endeguele Ekom
Research Unit of Microbiology and Antimicrobial Substances, Faculty of Science, University of Dschang, Dschang, Cameroon.
Michel Noubom
Department of Microbiology, Haematology and Immunology, Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Cameroon.
Josias Djenguemtar
Research Unit of Microbiology and Antimicrobial Substances, Faculty of Science, University of Dschang, Dschang, Cameroon and Département des Sciences Biomédicales et Pharmaceutiques, Faculté de Sciences de la Santé, Université Adam Barka d’Abéché, BP : 1173, Tchad.
Gabriel Tchuente Kamsu
Department of Human Biology, Faculty of Health Sciences, Walter Sisulu University, Mthatha, South Africa.
Huguette Bocanestine Laure Feudjio
Research Unit of Microbiology and Antimicrobial Substances, Faculty of Science, University of Dschang, Dschang, Cameroon.
Aurelie Dahlia Yemeli Piankeu
Research Unit of Microbiology and Antimicrobial Substances, Faculty of Science, University of Dschang, Dschang, Cameroon.
Louis-Claire Ndel Famen
Research Unit of Microbiology and Antimicrobial Substances, Faculty of Science, University of Dschang, Dschang, Cameroon.
Donatien Gatsing
Research Unit of Microbiology and Antimicrobial Substances, Faculty of Science, University of Dschang, Dschang, Cameroon.
Recent Global Research Developments in Understanding the Science of Diabetic Wound Healing
Diabetic Wound-Healing Science: This review discusses the pathophysiology of diabetic wounds, focusing on impaired angiogenesis, neuropathy, chronic inflammation, barrier disruption, and polymicrobial infection. It also explores current and future treatment strategies [1] .
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References
- Burgess JL, Wyant WA, Abdo Abujamra B, Kirsner RS, Jozic I. Diabetic Wound-Healing Science. Medicina. 2021; 57(10):1072. https://doi.org/10.3390/medicina57101072
- Keni, Raghuvir, Begum, Farmiza, Gourishetti, Karthik, Viswanatha, Gollapalle Lakshminarayanashastry, Nayak, Pawan Ganesh, Nandakumar, Krishnadas and Shenoy, Rekha R. “Diabetic wound healing approaches: an update” Journal of Basic and Clinical Physiology and Pharmacology, vol. 34, no. 2, 2023, pp. 137-150. https://doi.org/10.1515/jbcpp-2021-0340
- Simon Matoori et al. ,Advanced bandages for diabetic wound healing.Sci. Transl. Med.13,eabe4839(2021).DOI:10.1126/scitranslmed.abe4839
- Burgess, J.L.; Wyant, W.A.; Abdo Abujamra, B.; Kirsner, R.S.; Jozic, I. Diabetic Wound-Healing Science. Medicina 2021, 57, 1072. https://doi.org/10.3390/medicina57101072
