Deleterious SNPs of various genes have been identified using in silico methods and some of them include ATP-Binding Cassette transporter G2 (ABCG2), Ovastacin (ASTL), mammalian/mechanistic target of rapamycin (mTOR), Interleukin-10 (IL-10), mitochondrial tumor suppressor 1 (MTUS1), guanosine triphosphate cyclohydrolase 1 (GCH1), gap junction protein alpha 3 (GJA3), beta-defensin 1 (DEFB1), B-cell lymphoma/leukemia 11A (BCL11A) , and apoptosis protease-activating factor 1 (APAF1) . Observing and recording DNA polymorphisms in different genes and populations can help in developing personalized medicines .
Antimicrobial peptides (AMPs) are principal constituents of the innate immune system and have suppressive effects on bacteria, fungi, viruses, and parasites . Lysozyme C (LYZ C) is an important AMP secreted in body secretions such as milk, tears, and saliva. LYZ C cleaves β (1, 4) glycosidic bond linkage between N-acetyl muramic acid and N-acetyl glucosamine of the peptidoglycan layer of bacteria thereby causing loss of membrane integrity and leading to osmotic lysis of bacteria. As it is one of the important AMPs, polymorphisms in the LYZ C gene can reduce its antimicrobial potential and increase the susceptibility to infections.
Author(s) Details:
Harini Venkata Subbiah
Human Genetics Research Centre, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, 600100, India.
Usha Subbiah
Human Genetics Research Centre, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, 600100, India.
Recent Global Research Developments in Network-Based Detection of Single Nucleotide Polymorphism (SNP) Interactions
“Recent Progress in Single-Nucleotide Polymorphism Biosensors” – This review discusses improvements in SNP biosensing based on fluorescent and electrochemical methods[1] .
“Detection of Single Nucleotide Polymorphisms by Fluorescence Embedded Dye SYBR Green I Based on Graphene Oxide” – This research introduces a novel biosensor using SYBR Green I dye and graphene oxide for SNP detection [2] .
“Nanoparticle-based Detection and Quantification of DNA with Single Nucleotide Polymorphism Discrimination” – This study reports a gold nanoparticle-based method for DNA detection and SNP discrimination [3] .
“Single-Nucleotide Polymorphisms (SNP) Mining and Their Applications” – This article analyzes immune-related genes to identify SNPs through data mining [4] .
“Simultaneous Multiple Single Nucleotide Polymorphism Detection Based on DNA Ligation and Click Reaction” – This research mimics a ligation reaction for SNP detection using an enzyme-free DNA template-directed click reaction [5] .
References
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- Xia J, Xu T, Qing J, Wang L and Tang J (2021) Detection of Single Nucleotide Polymorphisms by Fluorescence Embedded Dye SYBR Green I Based on Graphene Oxide. Front. Chem. 9:631959. doi: 10.3389/fchem.2021.631959
- Wei Jie Qin, Lin Yue Lanry Yung, Nanoparticle-based detection and quantification of DNA with single nucleotide polymorphism (SNP) discrimination selectivity, Nucleic Acids Research, Volume 35, Issue 17, 1 September 2007, Page e111, https://doi.org/10.1093/nar/gkm602
- Vallejos-Vidal E, Reyes-Cerpa S, Rivas-Pardo JA, Maisey K, Yáñez JM, Valenzuela H, Cea PA, Castro-Fernandez V, Tort L, Sandino AM, Imarai M and Reyes-López FE (2020) Single-Nucleotide Polymorphisms (SNP) Mining and Their Effect on the Tridimensional Protein Structure Prediction in a Set of Immunity-Related Expressed Sequence Tags (EST) in Atlantic Salmon (Salmo salar). Front. Genet. 10:1406. doi: 10.3389/fgene.2019.01406
- Zhou, Q. Y., Yuan, F., Zhang, X. H., Zhou, Y. L., & Zhang, X. X. (2018). Simultaneous multiple single nucleotide polymorphism detection based on click chemistry combined with DNA-encoded probes. Chemical Science, 9(13), 3335-3340.