Spirometra Eggs Were Collected From Faeces of Experimentally Infected Cats : A Part from the Book Chapter : Study of Spirometra Egg by Scanning Electron Microscopy: Operculum and Operculum Suture at one End

Spirometra eggs were collected from faeces of experimentally infected cats. The eggs collected were used for morphology and measurement. Faeces of infected cats were collected with a scoop, placed in plastic bags, labelled and transported to the laboratory for processing. The faeces were placed in a beaker with tap water, and mixed well with a glass rod to break down the lumps. The mixture was sieved with sieves of mesh size 250, 150, 75, and 53 μm. The recovered eggs were washed with tap water and kept in bottles, stored in a fridge at 4ºC. To determine the presence of eggs in faeces the following procedures were carried out.

Author(s) Details:

Nicholas Jairo Kavana
Department of Microbiology and Parasitology, Faculty of Medicine, St. Francis University College of Health and Allied Sciences, Ifakara, Tanzania.

Also See : Several Subspecies of B. Thuringiensis have been Reported to Produce Chitinases : A Part from the Book Chapter : Genes chi of Native Bacillus thuringiensis Strains from Maranhão Active against Aedes aegypti Larvae

Recent Global Research Developments in Diagnostic Challenges in Spirometra Infection

Epidemiology, Diagnosis, and Prevention of Sparganosis in Asia:

This review provides comprehensive information on the life cycle, clinical characteristics, pathogenesis, and molecular diagnosis of Spirometra. It highlights the geographical distribution and infection characteristics of Spirometra in various hosts, which are crucial for preventing these parasites [1] .

Sparganosis (Spirometra) in Europe in the Molecular Era:

This article discusses the relatively neglected foodborne and waterborne disease caused by Spirometra species. It presents the first molecular evidence of the coincident presence of two species in Europe and reviews the current distribution to raise awareness of the parasite in this region [2] .

Presence of Spirometra mansoni, Causative Agent of Sparganosis, in South America:

This study reports the molecular identification of an adult Spirometra mansoni tapeworm retrieved from a crab-eating fox in Colombia, confirming the presence of this parasite in South America [3] .

Advances in Molecular Diagnosis of Spirometra Infection:

This research focuses on the advancements in molecular diagnostic techniques for Spirometra infection. It emphasizes the importance of DNA sequencing for reliable species identification, which is essential for understanding the origins and routes of spread of these parasites [1] .

Challenges in Diagnosing Sparganosis: A Review:

This review article addresses the diagnostic challenges associated with sparganosis, including the limitations of current diagnostic methods and the need for improved molecular techniques to accurately identify Spirometra species [2] .

References

1. Liu W, Gong T, Chen S, Liu Q, Zhou H, He J, Wu Y, Li F, Liu Y. Epidemiology, Diagnosis, and Prevention of Sparganosis in Asia. Animals. 2022; 12(12):1578. https://doi.org/10.3390/ani12121578
2. Roman Kuchta, Marta Kołodziej-Sobocińska, Jan Brabec, Daniel Młocicki, Rusłan Sałamatin, Tomáš Scholz, Sparganosis (Spirometra) in Europe in the Molecular Era, Clinical Infectious Diseases, Volume 72, Issue 5, 1 March 2021, Pages 882–890, https://doi.org/10.1093/cid/ciaa1036
3. Brabec J, Uribe M, Chaparro-Gutiérrez JJ, et al. Presence of Spirometra mansoni, Causative Agent of Sparganosis, in South America. Emerging Infectious Diseases. 2022;28(11):2347-2350. doi:10.3201/eid2811.220529.

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